The DuaLink NeoBento™ features 8 or 16 chips per plate. The DuaLink architecture has been specially designed to create fluidic isolation and co-culture of up to 2 cell types. Both channels are connected by microchannels to allow 2 independant cell cultures. Neurons are used as bio-sensors, cell bodies growing in one channel and extensions in microchannels to reach and innervate any organ cultivated in the opposite channel.
The NeuroFluidics Line by NETRI is fabricated in PDMS for high transparency and biocompatibility. This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics Devices are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection with up to 16 data points on the same plate. With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The DuaLink design of the NeuroFluidics Devices allows the culture of up to 2 cell types. It features 2 cell culture compartments each having its own media and coating solutions, connected through 1 compartment for fluidic isolation. Each of the 3 channels has one inlet and one outlet. In the DuaLink architecture, the lateral channels (1 and 3) are symmetric and the central channel (2) is smaller, to create a fluidic isolation compartment between them, enabling interaction while isolating the cellular compartment. Because of the micron scale of microchannels connecting channels, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1,2,3).
There are two formats available:
- LIGHT: to perform 8 individual experiments simultaneously (half-plate)
- FULL: to perform 16 individual experiments simultaneously (full plate)
The NeoBento format and technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays and more.
The DuaLink platform can be used for a wide variety of applications including innervated epithelial modeling, neuromuscular junction or toxicology and virology studies.
Content
1x DuaLink LIGHT or FULL
Specifications
| Parameters |
Channel 1 |
Channel 2 |
Channel 3 |
| Length (µm) |
18 800 |
6000 |
18 800 |
| Width (µm) |
1 000 |
200 |
1 000 |
| Height (µm) |
200 |
200 |
200 |
| Volume (µL) |
3.8 |
0.24 |
3.8 |
| Volume with inlets/outlets (µm) |
117.7 |
114.1 |
117.7 |
| Surface area (mm²) |
18.8 |
1.2 |
18.8 |
| Surface area with inlets/outlets (mm²) |
32.9 |
15.3 |
32.9 |
| Microchannels |
125x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm |
| Material |
Chip in PDMS, Structure in Polystyrene |
| Format |
Standard ANSI 96-well plate |
| Shel-life |
4 months after reception |
Documentation
DuaLink technical specifications
DuaLink application note - Improve reproducibility
DuaLink application note - Surface tension-based cell seeding
