SynRAM model is a complete platform to study the inflammation pathway in a realistic environment. Enabling real-time tracking of rolling, adhesion and migration processes, it recreates a histological slice of co-cultured tissue and/or tumor cells with a lumen of endothelial cells.
General
SynVivo's SynRAM model is designed to overcome the limitations of devices classically used in biology by taking into account fluid shear and size/topology observed in vivo.
The device allows to recreate the microenvironment and to easily model the transmigration phenomenon. By recapitulating a histological slice of co-cultured tissue and/or tumor cells with an endothelial cell lumen, the SynVivo platform provides a physiologically realistic model while allowing real-time monitoring of the rolling, adhesion, and migration processes.
- Mimic the physiological shear stress in a microvascular environment
- In vivo-like vascular morphology with fully enclosed lumen
- Cell-to-cell interaction is possible with co-culture
- Get real-time quantitative rolling, adhesion, and migration
This assay has been successfully validated against in vivo studies showing an excellent correlation with rolling speeds, adhesion patterns, and migration processes.
Starter Pack - To order the chips only, check our dedicated pages for microvascular and radial designs (ref SY-102008, SY-105001).
Examples of models developed using SynRAM
Real-time visualization of rolling, adhesion and migration
Real-time tracking of single-cell migration
Content
12x SynRAM chips (choice of IMN2 radial or SMN2 microvascular network chips)
1x Pneumatic priming device (required for priming tubing to remove air)
1x Manifold (allows for multiple devices)
1x Tygon Tubing .02"ID X .06"OD (100 ft)
25x Slide Clamps
50x Blunt Tip Needles
50x 1mL Syringes with Luer-Lok® Tip
This kit does not include the air pump needed to establish the air-liquid interface.
Specifications
| IMN2 radial |
SMN2 co-culture microvascular network
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- Outer channel (OC): 200 μm
- Tissue chamber: 1.8 mm diameter
- Depth: 100 μm
- Pillar spacing (SP): 8 µm
- Travel (T): 50 µm
- Pillar height: 100 µm
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Idealized co-culture chip microvascular network with pillar barrier.
- Pillar height: 3 µm
- Pillar diameter: 10 µm
- Separation: 50 µm
- Depth: 100 µm
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Documentation
?SynVivo brochure
?SynRAM idealized network technical manual
?SynRAM microvascular network technical manual
?SynVivo starter kit brochure
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Lamberti, G., Soroush, F., Smith, A., Kiani, M. F., Prabhakarpandian, B., & Pant, K. (2015). Adhesion patterns in the microvasculature are dependent on bifurcation angle. Microvascular research, 99, 19-25. https://doi.org/10.1016/j.mvr.2015.02.004
Lamberti, G., Prabhakarpandian, B., Garson, C., Smith, A., Pant, K., Wang, B., & Kiani, M. F. (2014). Bioinspired microfluidic assay for in vitro modeling of leukocyte–endothelium interactions. Analytical chemistry, 86(16), 8344-8351. https://doi.org/10.1021/ac5018716
Lamberti, G., Tang, Y., Prabhakarpandian, B., Wang, Y., Pant, K., Kiani, M. F., & Wang, B. (2013). Adhesive interaction of functionalized particles and endothelium in idealized microvascular networks. Microvascular research, 89, 107-114. https://doi.org/10.1016/j.mvr.2013.03.007
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