Field-flow fractionation chip

The Field-flow fractionation chip is a specialized platform designed for miniaturized free-flow electrophoresis, utilizing a chamber design with precise volume handling, selectable surface treatment options, and a single-channel configuration. This chip allows accurate setup and control of volume parameters for analytical separation.

General

The field-flow fractionation chip Fluidic 120 features olive interfaces and field-flow fractionation structure. The chip was developed within the BMBF-Project “Free-Flow-Chip”, FKZ 01RI0643D, and can be used to establish a miniaturized free-flow electrophoresis (FFE).  In free-flow electrophoresis, charged analytes entering the separation bed are deflected to the side by the electric field applied at right angles to the direction of flow, causing anions to migrate to the anode (positive pole) and cations to the cathode (negative pole). The combination of laminar flow and electrophoretic migration at a 90° angle to it forms a deflection angle, which depends on the electrophoretic mobility of the analyte in question and the applied flow rates and field strengths. Please note: This product only contains the plastic chip as platform to establish your experiment. It does not contain electrodes. For more information on miniaturized free-flow electrophoresis using this chip, check out our publication: S. Köhler et al. RSC Adv., 2012,2, 520-525 [https://pubs.rsc.org/en/content/articlelanding/2012/ra/c1ra00874a#!divAbstract].

Features & Benefits

• Miniaturized Platform: Enables the setup of free-flow electrophoresis in a compact, easy-to-handle format. Suitable for lab scale and research.
• Flexible Material Options: Available in both PC and Zeonor materials, allowing users to select based on chemical compatibility or physical needs.
• Surface Treatment Selection: Choice of hydrophilized or untreated surface enables control over wettability and interaction with fluids.
• Simple Integration: Olive interface connections support easy linking to standard laboratory tubing and fluid systems.
• Transparent Structure: Visual monitoring of flow and separation is straightforward due to fully transparent chip construction.
• No Embedded Membrane: Open channel design allows unobstructed flow and direct establishment of the electric field for separation.

This field-flow fractionation chip provides a dedicated solution for researchers aiming to establish free-flow electrophoresis on a miniaturized scale. The design gives maximum transparency into the setup process, ensuring accurate positioning and monitoring of the separation workflow. With support for different plastic materials and surface treatments, users can optimize experimental conditions for a broad range of analytes and applications.

By offering olive connector interfaces, the chip supports standard laboratory fittings, making it easy to incorporate into existing experimental systems. The chamber-based approach is optimized for controlled experiments requiring reliable electrophoretic separation. This chip is engineered for precise laboratory applications where flexibility in material and wettability is required.

Applications

The Field-flow fractionation chip is ideal for laboratory experiments involving miniaturized free-flow electrophoresis setups, separation of charged particles, protocol development, and chemical analysis where controlled fractionation is required.

• Miniaturized free-flow electrophoresis
• Separation of charged biomolecules
• Protocol optimization in flow-based chemical analysis

Content

1x Field-flow fractionation chip

Specifications