HUMIMIC Chip2 - 2-organ-chip

The HUMIMIC Chip2 is a cutting-edge organ-on-a-chip platform allowing the simultaneous culture of two tissue types in a single microfluidic circuit. Each chip displays 2 independent microfluidic designs. It provides a more realistic and dynamic model of human physiology. This dual-organ platform enables the study of complex inter-organ interactions, drug responses, and disease mechanisms in a controlled, miniature environment.

General

The HUMIMIC Chip2 allows the co-culture of up to 2 different tissues in a joint microfluidic circuit to develop Microphysiological Systems (MPS). The HUMIMIC Chips are operated via the HUMIMIC Starter, which can control several chips and chip types at the same time. Pressure and vacuum can be applied and allow the modeling of a lifelike supply of the tissues and also the modulation of shear stress according to the experimental needs of the user. The HUMIMIC Chip2 contains 3 pump membranes (500 µm thick) to control the liquid displacement in the microfluidic circuit, through valves activated by the pressure applied by the HUMIMIC Starter.

Each Chip2 device displays 2 separate circuits allowing to have two replicates per chip. The flexibility of the HUMIMIC device allows to study any organ and also biological barrier models like the intestine, the lung or the skin. It is compatible with cell line, primary cells but also biopsies, tissue sections or spheroids. It is also possible to use commercially available culture inserts or even matrix-suported cultures to mimick the 3D environment of the organs. These chips, made of polycarbonate, PDMS and glass, have been designed for long-term experiments allowing the study of disease models and drug's efficacy and toxicity.

The Chip2 devices exists in 2 formats, displaying different sizes of culture compartment to adapt to the studied organs and tissue types:

• The Chip2 24-well displaying one "96-well" and one bigger "24-well" (14 mm of diameter)
• The Chip2 96-well for which the two culture compartment are identical and equivalent to a 96-well (6.5 mm of diameter)

Each chip is provided with the relevant culture compartments which are screwed into the device thanks to a hexagonal socket wrench. For media exchange, the compartments of one circuit need to be opened separately.

The types of culture compartment proposed here are compatible with scaffolds and can also serve as reservoirs for up to 750 µL (Threadfit 03 for the Chip2-96 and Threadfit 03 and 05 for the Chip2-24). Note that the nature of the culture compartment can be changed according to what is described in the product catalog of the Documentation section.

All chips are provided prefilled with DPBS supplemented with 1% Penicillin/Streptomycin (PS) but can alos be provided with a mix of 0.01% Gentamicin / 0.1% Amphotericin B (GA) or without any antibiotics (WO) added in the DPBS. For any requests regarding customization, please contact us. 

Applications

The two chip models presented here have already been used for several applications:

• Chip2 24-well: 
  • Hair follicle
  • Intestine - Liver
  • Skin - Liver 
  • Skin - Tumor
  • Heart - Liver

• Chip2 96-well:
  • Bone marrow 
  • Pancreas - Liver 
  • Thyroid - Liver 
  • Bone 
  • Endometrium 

Content

1x HUMIMIC Chip2 (including cell culture compartments)

Specifications

Technical drawing of the Chip2 24-well

Technical drawing of the Chip2 96-well

Documentation

? HUMIMIC Chip2 24-well - Quick Guide

? HUMIMIC Chip2 96-well - Quick Guide