The Advanced Microfluidics LSPone+ is a high-volume, high-precision syringe pump designed for reliable, programmable liquid handling in microfluidics, analytical chemistry, and life science workflows. It shares the compact design and intuitive interface of the original LSPone, but supports larger syringe volumes (up to 5 mL), making it ideal for applications requiring longer runs or higher throughput.
Includes the LSPone+ pump unit, power supply, USB cable, LSPoneQuick software, glass syringe of your choice (2.5 mL or 5 mL), and syringe change tool. Fittings must be ordered separately.
General
The LSPone+ combines a high-precision syringe pump with a zero-dead-volume rotary valve in a compact and cost-effective system. It supports a wide flow rate range, from sub-microliter per minute to 150 mL/min, depending on syringe size. This makes it a robust solution for continuous flow applications, high-volume reagent dosing, and extended-duration perfusion experiments.
With its integrated multi-port valve, users can easily switch between liquids, automate sequences, and reduce cross-contamination risks. The included LSPoneQuick software allows precise control through an intuitive graphical interface or programmable scripts (Python, LabVIEW).
The pump is available in multiple port configurations to suit a variety of applications. The 10-port version with 1.0 mm channel diameter is ideal for higher flow rate needs, while models with the standard 0.5 mm channels better match typical microfluidic device dimensions.
Performance Overview
| Syringe volume |
Min. flow rate |
Recommended Min. flow rate* |
Max. flow rate |
Min. dosing volume |
| 2 500 µL |
0.373 µL/min |
50 µL/min |
75 mL/min |
5 µL |
| 5 000 µL |
0.745 µL/min |
250 µL/min |
150 mL/min |
10 µL |
*Flow rate variation may exceed 5% below the recommended minimum. For improved accuracy at lower flow rates, consider the LSPone+ HD version.
For smaller syringe volumes (50 µL to 1 mL), use the LSPone or LSPone HD versions, which are specifically designed for this range.
Advantages
- Combines high-precision syringe pump and multi-port rotary valve in one compact unit
- Supports 2.5 mL and 5 mL syringes for long-duration or high-throughput use
- Integrated rotary valve enables switching between multiple fluids
- Minimal carryover and internal volumes for better dilution control
- Bubble-free priming and near-pulseless flow
- LSPoneQuick GUI with scripting support (Python, LabVIEW)
- Swiss-made quality and excellent chemical compatibility
Applications
Sequential injection
The LSPone is ideal for automated sequential injections in multi-step chemical workflows. Its programmable functionality enables precise control over both timing and volume, making it well-suited for executing complex protocols with high reproducibility. Typical applications include surface functionalization, coating processes, and advanced techniques in spatial biology.
Volumetric injection
For applications demanding precise volumetric control, the LSPone ensures highly accurate liquid injections. This capability is especially valuable during sample preparation, where delivering exact reagent volumes is essential for consistency and performance. Common use cases include droplet microfluidics and microparticle synthesis (LNPs, EVs).
Dilution
The programmable control of the LSPone enables the automated preparation of complex mixtures and precise dilutions of samples or reagents. This streamlines workflows, enhances reproducibility, and significantly reduces the need for manual handling. Typical applications include gradient generation, buffer exchange, and sample preparation.
See the Documentation tab for Application Notes and Articles featuring the LSPone.
Special Offer
Get a special discount when purchasing 2 LSPone pumps together!
Content
1× LSPone+ body with rotary valve (PTFE/PCTFE)
1× Glass syringe (2.5 mL or 5 mL)
1× Syringe change tool
1× USB cable
1× Power supply with regional plug
LSPoneQuick software included
Note: Fittings are not included. See related products.
Specifications
| Max Pressure |
7 bars (102 psi) |
| Wetted Materials |
PTFE or UHMW‑PE, PCTFE, borosilicate glass |
| Syringe Size |
2.5 mL – 5 mL |
| Dead Volume |
None (zero-dead-volume valve) |
| Plunger Travel & Steps |
30 mm – 96,000 micro-steps |
| Accuracy |
< 1 % deviation full stroke |
| Stroke Time Range |
6 s – 6 400 min |
| Interface |
USB-mini, RS232/RS485 over USB |
| Power Consumption |
18–24 VDC, 2.2 A peak, 40 W |
| Dimensions (L×W×H) |
247×147×85 mm |
| Weight |
2.2 kg |
| Software/OS |
LSPoneQuick; Windows 10+ (also supports Python & LabVIEW) |
| Certifications |
CE & CB |
Note: Syringe size and valve port configuration must be selected when ordering. Performance may vary depending on fluid properties.
Valve specifications
| Valve configuration |
Zero dead-volume multi-port distribution with angular encoder |
| Number of ports |
6 |
10 |
12 |
| Channel diameter |
0.5 mm |
0.5 mm |
1.0 mm |
0.5 mm |
| Internal volume |
14.4 µL |
15.4 µL |
25.7 µL |
15.4 µL |
| Carryover volume |
13.4 µL |
13.6 µL |
18.6 µL |
13.6 µL |
| Tube port fittings |
Standard 1/4-28 UNF, flat-bottom |
| Dead volume |
None |
| Valve body material |
PTFE |
| Valve plug material |
PCTFE |
| Max. pressure |
7 bar (102 psi)
|
Documentation
LSPone brochure
Operating manual
Quick start guide
LSPoneQuick software manual
Maintenance guide
STEP file available on request.
Published Research using the LSPone
✅ High-throughput Proteomics Automation
In Yucel et al., 2024 (Nature Communications Biology), they developed a fully automated pipeline for preparing samples in proteomics. The LSPone helped automate fluid exchanges and sample transfers, reducing manual labor and improving consistency.
➕ Buffer exchange, sample prep, multi-step automation
→ Read the article
✅ Droplet Microfluidics & Microparticle Synthesis
Wang et al., 2024 (Digital Discovery) utilized the LSPone to control droplet formation and reagent injection in a machine-learning-guided synthesis platform for polymer microparticles. The pump’s precise volume control was key to reproducible particle generation.
➕ Droplet microfluidics, microparticle synthesis, precise dosing
→ Read the article
✅ Sample Prep for Immune System Analysis
In Li et al., 2023 – Cell Reports Methods (PubMed), they describe a microfluidic system for preparing tiny biological samples to study how the immune system recognizes disease. The LSPone helped automate the cleaning and preparation steps needed before analysis.
➕ Sample preparation, automated workflows, low-volume handling
→ Read the article
