The TriaLink MEA NeoBento™ features 8 chips per plate. The plate features a MicroElectrod Array (MEA) layer to record the activity of the neurons. The TriaLink MEA architecture has been specially designed to create fluidic isolation and co-culture up to 3 cell types. All channels are connected by microchannels to allow 3 independant cell cultures. Neurons are used as bio-sensors, cell bodies growing in channel and extensions in microchannels to reach and innervate any organs cultivated in the opposite channel, and record and process electrophysiological datas via MEA compatibility and NETRI's UpLink utility software.
The NeuroFluidics MEA Line by NETRI is fabricated in PDMS for high transparency and biocompatibility and is compatible with MEA-recording (Axion Maestro EDGE). This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics MEA Devices are MEA-capable compartmentalized organ-on-chip. They are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection, including electrophysiology, with up to 8 data points on the same plate. With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The TriaLink design of the NeuroFluidics Devices allows the culture of up to 3 cell types. It features 3 cell culture compartments each having its own media and coating solutions. Each of the 3 channels has one inlet and one outlet. Because of the micron scale of microchannels connecting channels, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1,2,3).
Thanks to the MicroElectrode Array (MEA) plate, developped in collaboration with Axion BioSystems, it is possible to record the electrical impact of compound on neurons somas or extensions. With 42 electrodes (white dots in the picture below) displayed both in the channels and microchannels, it is possible to record all neurons' parts: somas, axons and dendrites. It is also possible to monitor the propagation of an action potential from one channel to the other.
The electrophysiological recordings are performed thanks to Axion Biosystems' Maestro EDGE (8 chips per plate) instrument and results can be processed thanks to NETRI's Uplink software for easy interpretation of the collected data.
The plate comes in the EDGE format, displaying 8 independent designs (half-plate).
The technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays, Electrophysiology and more.
The TriaLink MEA platform can be used for a wide variety of applications including study the functional effect of probiotic on neurons via the gut-brain axis and many other applications.
Content
1x TriaLink MEA EDGE
Specifications
| Parameters |
Channel 1 |
Channel 2 |
Channel 3 |
| Length (µm) |
17 200 |
6000 |
17 200 |
| Width (µm) |
1 000 |
1000 |
1 000 |
| Height (µm) |
200 |
200 |
200 |
| Volume (µL) |
3.4 |
1.2 |
3.4 |
| Volume with inlets/outlets (µm) |
117.3 |
115.1 |
117.3 |
| Surface area (mm²) |
17.2 |
6 |
17.2 |
| Surface area with inlets/outlets (mm²) |
31.34 |
15.34 |
32.9 |
| Microchannels |
450x6(±1)x3,4 µm ; LxWxH n=200; spaced by 20 µm |
| Material |
Chip in PDMS, Structure in Polystyrene |
| Format |
Standard ANSI 96-well plate |
| Shel-life |
4 months after reception |
Documentation
TriaLink MEA technical specifications
Application note - Improve reproducibility
Application note - Surface tension-based cell seeding
