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The Open Membrane Chip - Direct access to membrane areas Fluidic 219 is a microfluidic device that provides direct, permanent access to membrane areas with flexible fluidic management. Its four independent membrane channels support a wide range of volumes. Volume selection and channel management are easily handled through supported fluidic interface systems, making the chip suitable for various filtering and assay tasks. It is designed for efficient supply, storage, or exchange of liquids—adaptable to specific experimental volume and channel needs in microfluidics research.
General
The open chip is designed to allow direct access to membrane areas and provide a permanent entry port for liquid supply, storage and exchange. It features 4 independent membranes. In combination with microfluidic ChipShop’s matching Interaction Tanks like Fluidic 234 and 235 for liquid supply and storage this chip allows for a wide variety of filtering and assay tasks. Interaction tank Fluidic 235 is equipped with a cap that includes Mini Luer fluidic interfaces to be easily connected to pumps for permanent operation.
This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.
Two different and independent culture chambers are available on the same chip. This allows you to perform two experiments in parallel or in series (body-on-a-chip).
The two chambers are designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each chamber indeed has multiple flow inlets and outlets for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.
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The chip comes with three options of PET membranes having different pore sizes. Choose between a 0.4 µm, 3µm or an 8 µm porous membrane.
Features & Benefits
- Direct access to membranes: The chip lets you work directly with each membrane. You have direct contact for liquid supply, storage or exchange. This gives you flexibility in experimental setup.
- Four independent membrane channels: Each chip contains four separate channels. You can run different liquids through each or use them together for parallel tasks.
- Compatible with interaction tanks: This chip matches Fluidic 234 and 235 tanks. Integration with tanks is straightforward—no custom adapters needed. Continuous operation is possible through Mini Luer interfaces.
- Clear construction and material options: Transparent materials help you watch your processes as they happen. Options for hydrophilized or untreated surfaces give you control over liquid interaction with membranes.
- Permanent entry port: The chip supports lasting setups without the risk of leaks or accidental disconnection.
- Mini Luer connection molded with the chip: leak-free
- Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer)
- Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
- Cover lid thickness: 140 µm
- 2 independent cell culture chambers
- Porous PET membrane: pore Φ of 0.4, 3, or 8 µm
Applications and examples from published papers
The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.
Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.
Intestine-on-chip
Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)
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3D microphysiological model of the human intestine.
In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.
Endothelial Barrier-on-chip
Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)
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The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.
They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.
Liver-on-chip
Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)
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The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.
During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.
Content
1x Open Membrane Chip - Direct access to membrane areas Fluidic 219
Specifications
| SKU |
CS-10001070 |
CS-10001071 |
CS-10001072 |
CS-10001069 |
| Design element |
Membrane |
Membrane |
Membrane |
Membrane |
| Material |
PS |
Topas |
PS |
Topas |
| Material chip body |
mcs-PS 17 |
Mcs-COC 13 |
mcs-PS 17 |
Mcs-COC 13 |
| Material chip lid |
mcs-foil 075 (PS; 125 µm) |
mcs-foil 011 (Topas; 140 µm; Tg 78°C) |
mcs-foil 075 (PS; 125 µm) |
mcs-foil 011 (Topas; 140 µm; Tg 78°C) |
| Color |
Transparent |
Transparent |
Transparent |
Transparent |
| Interface type |
Mini Luer interface |
Mini Luer interface |
Mini Luer interface |
Mini Luer interface |
| Surface treatment |
Not treated |
Hydrophilized |
Hydrophilized |
Not treated |
| Membrane |
mcs-membrane-115 (8 µm pore size; PET) |
mcs-membrane 075 (8 µm pore size) |
mcs-membrane-115 (8 µm pore size; PET) |
mcs-membrane 075 (8 µm pore size) |
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Documentation
Click to read more information about ChipShop chips material properties.
? General handling guide for cross-flow membrane chips (available soon)
? Cross-flow membrane chip with multiple ports Fluidic 653 datasheet
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043
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Documentation
Click to read more information about ChipShop chips material properties.
? Open membrane chip Fluidic 219 datasheet
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043
