"
The Cross-Flow Membrane Chip - Large membrane interaction area Fluidic 846 is a microfluidic device designed for advanced laboratory workflows that demand effective co-culture, filtration, or migration studies. Key features include a large membrane interaction area, flexible configuration of two chambers each with two inlets and one outlet, and robust membrane choice. This chip is ideal for experiments involving cell cultures, offering precision in fluid handling, multiple channel formats for experimental flexibility, and enables effective volume selection as needed for various protocols.
General
The cross-flow membrane chip Fluidic 480 features two chambers, which interact through a permeable membrane. Each chamber features two connected inlets and one outlet. Fluidic 846 can be used e.g. for cell culture experiments, where cells, such as epithelial cells are co-cultured. The chip is furthermore ideal for filtering or size exclusion or migration studies.
Features & Benefits
- Large membrane interaction area: Offers a broad surface for cell growth, filtration, or transport studies, increasing experimental accuracy and reproducibility.
- Dual chamber design: Each chamber has two inlets and one outlet. This supports complex experimental setups, such as co-culture or migration assays, and allows flexible flow arrangements.
- Versatile membrane options: Integrated PET membrane with 8 µm pores allows for direct interaction studies, filtration, or selective transport of cells and molecules.
- Flexible surface treatments: Available in hydrophilized or non-treated forms, accommodating various experimental needs, such as cell adhesion or pure size exclusion.
- Easy microfluidic connections: Mini Luer interfaces ensure fast and reliable connections to fluidic systems, increasing compatibility and ease of use.
- Transparent material: The chip materials are clear, making visual monitoring and microscope observation straightforward during experiments.
The Cross-Flow Membrane Chip - Large membrane interaction area Fluidic 846 gives researchers precision in setting up cross-chamber experiments. With its dual chamber layout and integrated membrane, it is an ideal platform to study cell interaction, migration, or molecular transport. The chip accommodates multiple surface treatments and material options, giving flexibility to a wide range of lab protocols.
This product is tailored for scientific work in cell biology, filtration tests, or pharmacological migration studies. It supports reproducible results and is simple to incorporate in standard microfluidic setups. Its sturdy build and compatible connections increase workflow efficiency and reliability in the lab.
Applications
The Cross-Flow Membrane Chip - Large membrane interaction area Fluidic 846 is specially made for use in research and development environments where fluidic separation, cell culture, or filtration are required. Its key applications include:
- Cell co-culture experiments (e.g., epithelial cell studies)
- Filtration and size exclusion analyses
- Cell migration investigations
Applications and examples from published papers
The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.
Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.
Intestine-on-chip
Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)
3D microphysiological model of the human intestine.
In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.
Endothelial Barrier-on-chip
Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)
The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.
They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.
Liver-on-chip
Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)
The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.
During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.
Content
1x Cross-Flow Membrane Chip - Large membrane interaction area Fluidic 846
Specifications
| SKU |
CS-10001122 |
CS-10001121 |
CS-10001123 |
CS-10001124 |
| Design element |
Membrane; Chamber |
| Material |
Topas |
PS |
| Material chip body |
Mcs-COC 13 |
mcs-PS 17 |
| Material chip lid |
mcs-foil 011 (Topas; 140 µm; Tg 78°C) |
mcs-foil 075 (PS; 125 µm) |
| Color |
Transparent |
| Interface type |
Mini Luer interface |
| Surface treatment |
Not treated |
Hydrophilized |
Not treated |
Hydrophilized |
| Membrane |
mcs-membrane 120 (8 µm pores; PET; transparent) |
Documentation
Click to read more information about ChipShop chips material properties.
? General handling guide for cross-flow membrane chips (available soon)
? Cross-flow membrane chip with large chamber Fluidic 846 datasheet
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043
