Reaction Chamber Chip - 50 µl chamber volume Fluidic 842

The Reaction Chamber Chip - 50 µl chamber volume Fluidic 842 is a versatile microfluidic device designed for reaction set-ups and cell culture. This chip supports a total chamber volume of 50 µl and features options for hydrophilized or untreated surfaces to suit a range of experiments. Multiple chamber formats are possible, allowing selection for specific workflows and protocols. Channel configurations are optimized for experimental flexibility and reproducibility.

General

Reaction chamber chips belong to the simplest, however most versatile, microfluidic devices. From basic reaction chamber to cell culture tool - many experimental setups can be facilitated with this chip. Fluidic 842 is particularly suited to be used for heat-cycle applications, such as PCR experiments, during which small air bubbles can form within the chambers of a microfluidic chip. For undisturbed fluorescent readouts, this microfluidic chip features microfluidic ChipShop‘s unique bubble-trapping-rim-design, specifically developed to keep the detection area of each chamber air bubble-free. Please make sure to select the right material and surface treatment for your particular experiment. For instance, for PCR applications with fluorescent readouts we recommend the use of Zeonor or PCR-suitable Topas, for cell culture experiments with adherend cells, we recommend the use of hydrophilized surfaces, which aid cell attachment.

Features & Benefits

• Bubble-Trapping-Rim: Stops air bubbles from entering the detection area during heat cycles. Improves the reliability of fluorescence signal readings in PCR.
• Surface Treatment Options: Offers both untreated and hydrophilized surfaces. Hydrophilized surfaces increase attachment of adherent cells for biology experiments.
• Material Choice: Available in Topas, Zeonor, and PC. Material selection allows optimal performance for both PCR and cell culture set-ups.
• Flexible Chamber Use: Use as a standard reaction chamber or for cell culture. Accommodates diverse experimental needs with the same chip design.
• Clear, Transparent Body: Enables consistent optical readout with minimal interference.

The Reaction Chamber Chip - 50 µl chamber volume Fluidic 842 is crafted specifically for flexible experimental set-ups in the microfluidics laboratory. Its bubble-trapping-rim avoids the disturbance of air bubbles during fluorescence-based heat cycling, which is essential for good PCR results. Users can select between untreated and hydrophilized surfaces to match application needs, such as cell attachment or nucleic acid amplification.

You are free to choose among different chip materials, which provide best results for particular lab protocols. Zeonor and PCR-grade Topas are suggested for fluorescence PCR, while hydrophilized surfaces support cell-based studies. This product allows you to tailor both the chemistry and surface for the best result in each workflow.

Applications

This chip is ideal for microfluidic experiments requiring a controlled chamber volume, including:

• PCR and other heat cycle reactions
• Cell culture studies, especially with adherent cells
• General biochemical assays and reaction kinetics

Content

1x Reaction Chamber Chip - 50 µl chamber volume Fluidic 842

Specifications

Documentation

Reaction Chamber Chip - 50 µl chamber volume Fluidic 842 datasheet

Check the chemical compatibilities for this product here:
Topas - Chemical Compatibility Chart