Field-flow fractionation chip Fluidic 159

The Field-flow fractionation chip - Fluidic 159 is a specialized microfluidic device designed to enable miniaturized free-flow electrophoresis (FFE) experiments. Featuring olive fluidic interfaces and a dedicated chamber structure, this chip supports precise separation tasks. Volume selection is determined by the experimental setup, and the platform is engineered for operation as a single channel system, supporting high reproducibility and controlled fractionation processes.

General

The field-flow fractionation chip Fluidic 159 features olive interfaces and field-flow fractionation structure. The chip was developed within the BMBF-Project “Free-Flow-Chip”, FKZ 01RI0643D, and can be used to establish a miniaturized free-flow electrophoresis (FFE).  In free-flow electrophoresis, charged analytes entering the separation bed are deflected to the side by the electric field applied at right angles to the direction of flow, causing anions to migrate to the anode (positive pole) and cations to the cathode (negative pole). The combination of laminar flow and electrophoretic migration at a 90° angle to it forms a deflection angle, which depends on the electrophoretic mobility of the analyte in question and the applied flow rates and field strengths. Please note: This product only contains the plastic chip as platform to establish your experiment. It does not contain electrodes. For more information on miniaturized free-flow electrophoresis using this chip, check out our publication: S. Köhler et al. RSC Adv., 2012,2, 520-525 [https://pubs.rsc.org/en/content/articlelanding/2012/ra/c1ra00874a#!divAbstract].

Features & Benefits

• Miniaturization: Enables experiments on a small scale, saving sample and reagents.
• Dedicated FFE Structure: Designed for optimal separation of charged analytes in free-flow electrophoresis tasks.
• Flexible Interface: Olive fluidic interfaces offer reliable connections and easy integration with lab setups.
• High Transparency: Made from clear polymers for visual inspection during experiments.
• Surface Treatment Options: Available in both hydrophilized and untreated surface finishes to support different protocols.
• Chamber Design: Chamber element maximizes control over field-flow and analyte movement.

This chip is intended for R&D or laboratory users who want to establish miniaturized free-flow electrophoresis. Users can precisely control analyte migration using adjustable voltage and flow. The robust plastic design makes it suitable for repeated experimental use. It is compatible with both PC and Zeonor chip architectures, offering flexibility for different research needs.

By providing only the chip as a platform, users can customize their experiments with their own electrodes and peripheral devices. The olive interface ensures a secure connection to typical lab tubing, and the device is transparent for straightforward observation. The chamber geometry and available surface treatments help support both standard and challenging applications.

Applications

The Field-flow fractionation chip - Fluidic 159 is ideal for:

• Miniaturized free-flow electrophoresis experiments
• Separation of charged analytes
• Laboratory and research method development
• Microfluidic proof-of-concept experiments
• Process optimization for analytical separation

Content

1x Field-flow fractionation chip - Fluidic 159

Specifications

Documentation

Field-flow fractionation chip Fluidic 159 datasheet

Check the chemical compatibilities for this product here:
Polycarbonate - Chemical Compatibility Chart