PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 675

The PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 675 is a PCR chamber chip that divides one microfluidic stream into eight separate reaction chambers, each with 20 µl volume. You can choose the surface treatment option for flexibility. The unit has 8 channels in total. This chip allows precise volume buffering and prevents reagent loss during thermocycling, making it unique for applications that require secure, efficient multi-chamber PCR processing.

General

These PCR chamber chips with splitter function are particularly well-suited for applications in which one microfluidic stream needs to be divided into several fractions for downstream applications. In Fluidic 675, the input is divided into eight separate 20 µl reaction chambers. Each reaction chamber is succeeded by a chamber containing an enclosed air volume, which serves to buffer volume expansion/contraction during thermocycling. Because this air volume is sealed, there is no reagent loss due to evaporation. Furthermore, Fluidic 675 features microfluidic ChipShop's unique bubble-trapping rim design, specifically developed to maintain the detection area of each chamber free from air bubbles for undisturbed fluorescent readouts. This feature makes the chip particularly suitable for use in PCR experiments, during which small air bubbles can form within the chambers of a microfluidic chip. The loading pressure is designed for 250 mBar.

Features & Benefits

• Divides Input Stream: Splits one microfluidic stream into eight reaction chambers efficiently. Simple input split improves workflow for downstream PCR and related processes.
• Dead-End Air Reservoirs: Each chamber has an enclosed air volume. This buffers thermal expansion or contraction and prevents loss from evaporation during PCR cycles.
• Bubble-Trapping Rim: Design keeps detection areas free from bubbles. This is important for clear fluorescent readouts, ensuring results are reliable.
• Surface Treatment Options: Choose hydrophilized or not treated, depending on the assay requirements. This makes the product flexible and suitable for various protocols.

Fluidic 675 is optimized for microfluidic applications that demand precise aliquoting into multiple chambers. The chip's enclosed air volumes maintain a stable environment during PCR thermocycling, supporting accurate and reproducible assays. Its unique rim design reduces detection noise by keeping air bubbles from interfering with optical measurements, a frequent concern for high-sensitivity fluorescent assays. Each split chamber maintains the 20 µl standard volume, making the chip suitable for parallel assays or multiplexed reactions in research or diagnostic contexts.

The choice of hydrophilized or not treated surface gives users additional control over liquid handling properties and compatibility with different molecular biology protocols. These features are combined in a robust, transparent Topas polymer construction, providing a reliable, easy-to-handle microfluidic platform for demanding laboratory environments.

Applications

This chip is ideal for multiplex PCR, high-throughput screening, and experiments where one sample needs splitting into many reactions. Suitable for research, clinical diagnostics, and quality control in life science laboratories.

• Parallel PCR reactions for DNA/RNA analysis
• Multiplexing assays in molecular biology labs
• Sample splitting for high-throughput neonatal screening

Content

1x PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 675

Specifications

Documentation

PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 675 datasheet

Check the chemical compatibilities for this product here:
Topas - Chemical Compatibility Chart